ABSTRACT
OBJECTIVE：To investig ate the effects of tenuifolin （TEN）on brain mitochondrial autophagy in Aizheimer ’s disease（AD）model mice. METHODS ：Totally 50 male APP/PS1 double transgenic mice were randomly divided into model group，TEN medium-dose+ 3-MA group [TEN 40 mg/（kg·d）+autophagy inhibitor 3-MA 30 mg/（kg·d）] and TEN low-dose ， medium-dose and high-dose groups [ 20，40，80 mg/（kg·d）]，with 10 mice in each group. In addition ，10 wild-type homologous mice were included in normal control group. Administration groups were intragastrically given corresponding drug solution ；normal control group and model group were intragastrically given 0.3％ sodium carboxymethyl cellulose solution ，once a day ，0.01 mL/g， for consecutive 3 months. After last administration ，positive expression [measured by integrated optical density （IOD）] of microtubule associated protein 1 light chain 3（LC3）in neuron was detected ；mRNA expressions of LC3，ubiquitin-binding protein p62，Cathepsin D ，Rab7，phosphatase and tensin homolog deleted on chromosome ten gene-induced putative kinase 1（PINK1） and E 3 ligase（Parkin）as well as protein expressions of LC 3，p62，PINK1 and Parkin were detected in brain mitochondria. RESULTS：Compared with normal control group ，IOD value of LC 3 in neuron as well as mRNA and protein expressions of LC 3， p62，PINK1 and Parkin in brain mitochondria were all increased significantly in model group （P＜0.05 or P＜0.01），while mRNA expressions of Cathepsin D and Rab 7 were decreased significantly （P＜0.05 or P＜0.01）. Compared wit h model group ，IOD values of LC 3（except for TEN low-dose and medium-dose groups ） in neuron ，mRNA expressions of LC 3，Cathepsin D ，Rab7， PINK1（except for TEN low-dose group ）and Parkin （except for TEN low-dose group ） in brain mitochondria as well as protein expressions of LC 3 （except for TEN medium-dose group），PINK1（except fo r TEN high-dose group decreased significantly）and Parkin （except for TEN low-dose group decreased significantly ）were increased significantly in TEN low-dose ， medium-dose and high-dose groups （P＜0.05 or P＜0.01）；mRNA（except for TEN low-dose group ）and protein expressions of p62 were decreased significantly （P＜0.05 or P＜0.01）. Compared with TEN medium-dose group ，the changes of above indexes were inhibited significantly in TEN medium-dose + 3-MA group （P＜0.05 or P＜0.01）. CONCLUSIONS ：TEN can induce mitophagy in brain tissue of AD model mice by activating PINK 1/Parkin signaling pathway and improve lysosome function.
ABSTRACT
Objecitve To explore the relationship between basal blood pressure levels and prognosis in patients with hemorrhage,and the influence of different blood pressure control strategies in hematoma expansion and bleeding again.Methods Research methods for group-control,46 patients with hemorrhage from January 2010 to January 2013 according to the basal blood pressure levels (systolic blood pressure) in the first day of admission were divided into group A (＜ 180 mmHg,1 mmHg =0.133 kPa) 7 cases,group B (180-200 mmHg) 6 cases and group C (＞200 mmHg) 33 cases.At the same time according to different blood pressure control strategies were divided into group E (mean arterial pressure decreased ≥ 20％) 31 cases and group F (mean arterial pressure decreased ＜ 20％) 15 cases,the prognosis among different groups was analyzed.Results The 6 months death / disability rate in group C was higher than that in group A and group B [21.2％ (7/33) vs.1/7,1/6],there was significant difference (P ＜ 0.05).There was no significant difference between group A and group B(P＞ 0.05).Group E patients occurred with hematoma expansion in 1 case,group F occurred with hematoma expansion in 4 cases,and bleeding again in 1 case,incidence of adverse events in group E was lower than that in group F [3.2％ (1/31) vs.5/15],there was significant difference (x2 =5.642,P =0.018).Conclusion Early onset of high blood pressure (＞ 200 mmHg) is the independent risk factor of prognosis,and early actively antihypertensive can effectively reduce the occurrence of hematoma expansion and bleeding again.
ABSTRACT
Aim To study the effect of active components of isoflavones on PC12 cells damage induced with A?_(1-42). Methods The active components of isoflavones were extracted. Using PC12 cells damage induced by A?_(1-42) as the model,the protective effect of active components was determined with morphological examination and MTT and LDH assays. Results The morphological change of the damaged PC12 cells was improved, and the survival rate of the cells was significantly increased(P
ABSTRACT
The wild type cells of human stomach glandular carcinoma, cell line BGC 823, were treated firstly by a chemical carcinogen (MNNG) for the induction of mutagenesis, then the cells were selected in medium with gradually increasing amount of 8-AG (8-azaguanine) from 1-20?g/ml. It was found that the mutant cells could grow vigorously in the medium containing 20?g/ml of 8-AG but not in HAT medium. The HGPRT assay showed an obvious quantitative difference between the wild type and HGPRT mutant cells of BGC 823.